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1x phosphate buffered saline  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x phosphate buffered saline
    1x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+pbs/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pmc13088972-371-15-21
    Average 99 stars, based on 1 article reviews
    1x phosphate buffered saline - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Mechanically Programmable DNA Hydrogel Microparticles for 3D Cellular Systems.
    Article Snippet: .. The small peptide cyclo[Arg-Gly-Asp-D-Phe-Lys(Azide)] (c[RGD], Hölzel Diagnostika Handels GmbH) was diluted in a 1x PBS (no CaCl2, no MgCl2, pH = 7.4; Gibco) solution to yield a concentration of 2.4 mM. ..

    Incubation:

    Article Title: Cutibacterium acnes induce T cell extracellular traps with distinct proteome programs.
    Article Snippet: .. 1X PBS was aspirated and cells were resuspended and incubated in the dark for 30 min at 37°C and 5% CO2 in a Live Cell Imaging Solution (Invitrogen) cocktail containing 10 μg/mL Hoechst 33342 (ThermoFisher Scientific) and 20 nM Memglow 590 (Cytoskeleton). ..

    Article Title: MiR-940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes.
    Article Snippet: .. In + STA conditions, 20 h after transfection, 1 μM staurosporine was added to the cultures for 4 h. 24 h after transfection, media was removed from all wells before being washed with 1x PBS and then incubated in 1 mL Accutase (Gibco) for 5 min at 37◦C. .. Lifted cells were then collected in media and centrifuged at 300 x g for 3 min, washed with 1x PBS, and again centrifuged at 300 x g for 3 min. AnnexinV assays were performed using the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, Cat # 640922), according to the kit protocol.

    Article Title: Nodule development in Medicago truncatula is promoted by EPIDERMAL PATTERNING FACTOR-LIKE peptides.
    Article Snippet: Article Nodule development in Medicago truncatula is promoted by EPIDERMAL PATTERNING FACTOR-

    Article Title: Methamphetamine potentiates the use of outcome-specific associations via a hypothalamic-dopamine circuit.
    Article Snippet: Sections were washed with 1X PBS another 3 times for 15 minutes before incubating in blocking solution containing rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA; AB152) for 48 hours at 4oC. .. After primary incubation, sections were washed with 1X PBS another 3 times for 30 minutes before secondary incubation in blocking solution containing goat anti-rabbit IgG (H+L), Alexa FluorTM ll OPEN ACCESSArticle Neuron 114, 1–22.e1–e9, October 21, 2026 e7 488 conjugate (1:500, Thermo Fisher Scientific; A11008) or goat anti-rabbit IgG (H+L), Alexa FluorTM 594 conjugate (1:500; Thermo Fisher Scientific; A11012) for 2 hours at room temperature. .. Sections were washed another 3 times with 1X PBS before being incubated in DAPI (4’,6’-Diamidino-2-Phenylindole, Dihydrochloride; 1:10000; Thermo Fisher Scientific; D1306) made up in di H2O to stain for nuclei for 30 minutes.

    Live Cell Imaging:

    Article Title: Cutibacterium acnes induce T cell extracellular traps with distinct proteome programs.
    Article Snippet: .. 1X PBS was aspirated and cells were resuspended and incubated in the dark for 30 min at 37°C and 5% CO2 in a Live Cell Imaging Solution (Invitrogen) cocktail containing 10 μg/mL Hoechst 33342 (ThermoFisher Scientific) and 20 nM Memglow 590 (Cytoskeleton). ..

    Transfection:

    Article Title: MiR-940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes.
    Article Snippet: .. In + STA conditions, 20 h after transfection, 1 μM staurosporine was added to the cultures for 4 h. 24 h after transfection, media was removed from all wells before being washed with 1x PBS and then incubated in 1 mL Accutase (Gibco) for 5 min at 37◦C. .. Lifted cells were then collected in media and centrifuged at 300 x g for 3 min, washed with 1x PBS, and again centrifuged at 300 x g for 3 min. AnnexinV assays were performed using the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, Cat # 640922), according to the kit protocol.

    Blocking Assay:

    Article Title: Methamphetamine potentiates the use of outcome-specific associations via a hypothalamic-dopamine circuit.
    Article Snippet: Sections were washed with 1X PBS another 3 times for 15 minutes before incubating in blocking solution containing rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA; AB152) for 48 hours at 4oC. .. After primary incubation, sections were washed with 1X PBS another 3 times for 30 minutes before secondary incubation in blocking solution containing goat anti-rabbit IgG (H+L), Alexa FluorTM ll OPEN ACCESSArticle Neuron 114, 1–22.e1–e9, October 21, 2026 e7 488 conjugate (1:500, Thermo Fisher Scientific; A11008) or goat anti-rabbit IgG (H+L), Alexa FluorTM 594 conjugate (1:500; Thermo Fisher Scientific; A11012) for 2 hours at room temperature. .. Sections were washed another 3 times with 1X PBS before being incubated in DAPI (4’,6’-Diamidino-2-Phenylindole, Dihydrochloride; 1:10000; Thermo Fisher Scientific; D1306) made up in di H2O to stain for nuclei for 30 minutes.



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